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Image Search Results
Journal: ACS nano
Article Title: Kinetic Control in Assembly of Plasmid DNA/Polycation Complex Nanoparticles
doi: 10.1021/acsnano.9b03334
Figure Lengend Snippet: (A) In vitro transfection efficiencies of nanoparticles (W1–W8, see Table 1) in PC3 cancer cell line (dose = 0.6 μg gWiz-Luc plasmid/104 cells); (B) In vivo transfection efficiency in the lung in healthy BALB/c mice at 12 h post i.v. injection of nanoparticles (W1–W8, see Table 1) containing 40 μg gWiz-Luc plasmid per mouse (left) and representative IVIS images of groups with significant differences in transgene expression (right); (C) In vivo transfection efficiency in the lung of an LL/2 metastasis model in the NSG mice at 48 h post injection of nanoparticles (P1-P8, see Table 1) containing 40 μg PEG-Luc plasmid per mouse (left) and representative IVIS images of groups with significant differences in transgene expression (right); (D) Whole-body biodistributions in BALB/c mice at 1 h post injection of nanoparticles (W1, W2, W6, W8) containing 40 μg 3H-labeled gWiz-Luc plasmid per mouse. Labels: H: heart, K: kidneys, S: stomach, SI: small intestine; (E) Biodistributions to the lung of mice shown in (D); For statistical analysis, n.s. denotes no statistical significance with p > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001 from one-way or two-way ANOVA and multiple comparisons.
Article Snippet: Preliminary tests revealed that the transgene expression level (luciferase concentration in healthy lung tissues or tumor cells in the lung) peaks at around 12 and 48–72 h post injection for healthy BALB/c mouse model (Jackson Laboratory, US) and LL/2 metastasis in
Techniques: In Vitro, Transfection, Plasmid Preparation, In Vivo, Injection, Expressing, Labeling